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Illumina Inc
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Roche
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Complete Genomics Inc
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Image Search Results
Journal: GigaScience
Article Title: StereoSiTE: a framework to spatially and quantitatively profile the cellular neighborhood organized iTME
doi: 10.1093/gigascience/giae078
Figure Lengend Snippet: Spatial transcriptomic mapping of xenograft model in situ . (A) Flowchart illustrating the construction of the xenograft model is presented. BALB/c mice were subcutaneously injected with CT26 cells derived from colon cancer. Subsequently, the mice were either treated with a vehicle or DMXAA for a period of 14 days posttumor transplantation. Tissue samples were collected 24 hours after the treatment. (B) Heatmap depicting the expression of transcriptional markers of 11 annotated immune cell types at a single-cell resolution is shown. (C) An analysis of the proportion of immune cells at a single-cell resolution in each sample is provided. (D) Histogram comparing nonimmune cells to immune cells is displayed, where the blue bar represents the proportion of nonimmune cells, and the gray bar represents the integrated proportion of immune cells. The numbers associated with each bar indicate the precise cell counts for each sample. (E) Representative hematoxylin and eosin staining images of sample 717 from the control group (left) and sample 718 from the treatment group (right) are presented. The red-thread restricted region highlights the necrotic site. (F) In situ visualization of annotated cell types using stereo-seq data is shown on the left, an enlarged image of the marked-circle-site displaying cellular compositions is in the middle, and representative immunohistochemistry staining (CD163 indicating macrophages in sample 717 and Ly6G indicating neutrophils in sample 718) of the same marked-circle-site is presented on the right.
Article Snippet: The prepared section underwent processing in accordance with the Stereo-seq Transcriptomics Set User Manual (STOmics), utilizing reagents from the Stereo-seq Transcriptomics T kit and
Techniques: In Situ, Injection, Derivative Assay, Transplantation Assay, Expressing, Staining, Control, Immunohistochemistry